role of protein microarray in drug discovery slideshare

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Looks like you’ve clipped this slide to already. Other researchers use 5% phenol in ethanol which achieves the same result, prior to RNA extraction and purification. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Drug metabolism in pharmaceutical research has traditionally focused on the well-defined aspects of absorption, distribution, metabolism and excretion, commonly-referred to ADME properties of a compound, particularly in the areas of metabolite identification, identification of drug metabolizing enzymes (DMEs) and associated metabolic pathways, and reaction mechanisms. The principle aim of using microarray technology as a gene expression profiling tool is to answer some of the fundamental questions in biology such as "when, where, and to what magnitude genes of interest are expressed. You can change your ad preferences anytime. Each pin will spot a different grid on the array (Chapter 4). Cell communication and signaling ,cell cycle, No public clipboards found for this slide, Role of nuclicacid microarray &protein micro array for drug discovery process. They can be used for studying … •A protein microarray (protein chip)is a high throughput method used to track the interactions & activities of proteins,& to determine their functions,& determining function on a large scale. Although nucleic acids may also be considered, their use as drug targets in drug discovery and structure based drug design has been limited due to various effects like toxicity, difficulty in achieving high specificity, etc. INTRODUCTION • Protein micro array(or Protein chip) is a high- throughput method used to track the interactions & activities of proteins, to determinate their functions. The spots are printed on to the glass slide by a robot or are synthesised by the process of photolithography. Abstract: Protein microarray technology is one of the most powerful tools presently available for proteomic studies. Now customize the name of a clipboard to store your clips. role of nuclic acid microarray and protein microarray for drug discovery process 1.introduction about microarray technique and genomics 2.process of drug disco… Information from newer microarray technologies such as CGH, ChIP-on-chip, splice variants and microRNAs, combined with gene expression data, can be applied to the drug discovery process enabling many exciting applications. 4.Toxicological research A rapid platform for the research of the impact of toxin on the cells and their passing on to the progeny. Looks like you’ve clipped this slide to already. Microarrays for gene expression have made a profound impact in the pharmaceutical and biomedical worlds. A single-wavelength laser beam scanned back and forth across the array to excite an area representing a single pixel. Microarray technology uses chips with attached DNA sequences as probes for gene expression. Today drug discovery involves screening hits, medicinal chemistry, and optimization of hits to reduce potential drug side effects (increasing affinity and selectivity). (b) The pins are held in a cassette in a rectangular grid, which in turn is held on a robot arm that can be moved between the microtiter well plates and the glass arrays to deposit liquid. 2, No. ROLE OF TARGET IDENTIFICATION AND TARGET VALIDATION IN DRUG DISCOVERY Presented by:- Pallavi Duggal M.Pharmacy (2nd Semester) Department of Pharmacology ISF … Protein microarrays Martyna Franczuk Functional protein microarrays differ from analytical arrays in that functional protein arrays are made of full-length functional ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 56be2a-YmNmY Photobleaching is the destruction of a fluorophore by high intensity light, resulting in reduced signal intensity and consequent underestimation of expression level. Clipping is a handy way to collect important slides you want to go back to later. Protein microarrays are mainly applied to protein function studies, screening the production of antibodies and recombinant proteins , discovery of proteins implicated in disease or those that are potential drug targets, rapid detection or diagnosis of disease (18,19) screening for protein–protein, DNA–protein and enzyme–substrate interactions (20,21). Identifying the drug target The majority of available drugs have protein molecules as their targets. Microarrays are widely used to address a plethora of scientific questions in the pharmaceutical industry, particularly in drug discovery and development. Functional Protein Microarrays in Drug Discovery. Clipping is a handy way to collect important slides you want to go back to later. Important for toxicogenomic studies. GPCRs are successfully used as drug targets because the binding of natural ligands to their paired GPCR(s) can be moderated by appropriate small molecule drugs 1., 2..Effective engineering of these drugs is essential, however, because aberrant binding to such a physiologically significant target class can lead to serious side effects. This presentation will take you through the concept of microarray and its application in detail. Genetic aging Drugs Environment Cell Figure 1. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Protein Expression for MicroArrays, H.H. Efficacy or potency, metabolic stability (half-life), and oral bioavailability are also improved in this step of the drug development process. They are essential for protein folding and stability and act as recognition elements in cell–cell and cell–matrix interactions. Drug discovery Applicable in pharmacogenomics Comparative analysis of gene Help the identification of specific proteins produce by diseased cells. Microarrays play an increasingly significant role in drug discovery. ... 1.3.2.2 DnA microarrays Applications in Drug Discovery and Development Taken together, these studies revealed that interesting proteins identified by proteomics in the intracellular pathways targeted by anticancer drugs can be classified into several categories, including proteins related to mitochondrial function, endoplasmic reticulum (ER) stress proteins, oxidative stress and heat shock proteins, and nuclear proteins involved in signaling transduction. Role of Target Identification and Target Validation in Drug Discovery Process 1. The physiological state of a cell is defined by external and internal parameters. The drug discovery process was beginning in 19 th century by John Langley in 1905 when he proposed the theory of respective substances. Protein microarrays allow for the simultaneous and rapid analysis of thousands of proteins in high-throughput (HT). 3. DOI link for Functional Protein Microarrays in Drug Discovery. Harwood Emerging Trend: Cell-Free Protein Expression, F. Katzen and W. Kudlicki Fabrication of Functional Protein Microarrays The Critical Role of Surface Chemistry in Protein Microarrays, A. Guo and X.-Y. Now customize the name of a clipboard to store your clips. DNA Microarrays are one of the most effective invention ever developed. (d) Most pins in modern use have a reservoir that holds sample and so can print multiple features – usually on different arrays – from a single visit to the well containing probe. The technique has immense potential and promises to play a key role in furthering research in a number of fields, as discussed in this chapter. abnormal proteins may appear which will ultimately affect the other functions ... pharmacogenomics can play a major role in the treatment of patient, with minimised side effects. Yim, T.G. 11, pp. role of nuclic acid microarray and protein microarray for drug discovery process 1.introduction about microarray technique and genomics 2.process of drug discovery 3.microarray techiques 4.microarray analysis in drug discovery 5.steps involved in the micro array analysis. DNA microarrays are … We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. (c) The number of pins in the cassette can vary. Pre-hybridisation of microarrays is a valuable step for reducing non specific hybridisation. An update on using protein microarrays in drug discovery. The Critical Role of Surface Chemistry in Protein Microarrays . RNAprotect™ (Ambion) and RNAlater™ (Qiagen) are commercially available solutions that perfuse cells, and ‘freeze’ the cellular RNA profile. PROTEIN MICROARRAYS Protein microarray is an emerging technology that provides a versatile platform for characterization of hundreds of thousands of proteins in a highly parallel and high-throughput way. Principles of Clinical Pharmacology NIH, April 26, 2007 Role of FDA in Guiding Drug Development Carl Peck Center for Drug Development Science UCSF, UC-Washington Center – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 52a023-MjJmM Protein Microarrays By Drug Discovery Trends Editor | November 12, 2007 SomaPlex slides from Protein Biotechnologies, for screening protein biomarkers in human tumors, are arrays prepared from a vast collection of total protein lysates derived from numerous human tissues representing a range of cancer subtypes. Chappell, and S.H. Department of Chemistry and Chemical Biology, and Bauer Center for Genomics Research, ... it is discovery-oriented because unknown proteins, as well as known proteins, The PMT amplifies the signal from each photon of light, which is converted into a digital value used to create the image representing signal intensity at each pixel position. If you continue browsing the site, you agree to the use of cookies on this website. Proteomic approach of drug discovery includes finding an unstable protein that is causing an undesirable Thus, being at the heart of medically relevant biological processes, glycans have come onto the scene and are considered hot sp Sodium chloride and sodium citrate (SSC) as a source of monovalent cations, these interact mainly with the phosphate groups of the nucleic acids decreasing the electrostatic interactions between the two strands. Probe labelling can be achieved either by direct incorporation of Cy3 or Cy5 UTP into the reverse transcription reaction or by incorporation of UTP containing an allyl amino group followed by subsequent coupling of Cy dye. Glycans are key players in many biological processes. Microbial deterioration of textiles and paper, Customer Code: Creating a Company Customers Love, No public clipboards found for this slide. An example of a spotting robot. PROTEIN MICROARRAY K.KRUPA SAGAR M.Pharm 1st year 2. microarra y as a gene expression profiling tool microarra y as a comparativ e genomics tool disease diagnosis drug discovery toxicologic al research 22. Recent advances in proteomic technologies, including both mass spectrometry and protein microarrays, have enabled large-scale screening of proteins in tissues and serum from patients that are applicable for biomarker discovery. Attractive research tools because they enable researchers to study multiple protein interaction events and activity networks simultaneously. Protein microarrays for drug candidate profiling. Written by a leader in the field, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. Earlier robots use solid pins, which can only print one feature before needing to collect more DNA from the well. See our User Agreement and Privacy Policy. http://www.bio.davidson.edu/courses/genomics/chip/chip.html. The more pins, the greater the throughput of the robot, but the greater the propensity for pin-to-pin variability. Here, I discuss the role of microarray technology in this burgeoning area. If you continue browsing the site, you agree to the use of cookies on this website. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. See our User Agreement and Privacy Policy. (2007). See our Privacy Policy and User Agreement for details. Microarray technology for genomic and proteomic research. Protein microarray (protein arrays or protein biochips) technology is a process of immobilizing numerous proteins on to a solid support and using miniaturized assays to describe protein interactions, that has been developed steadily in recent years. There are many different robots on the market; this is a Genetix spotting robot located at the Mouse Genetics Unit in Harwell, Oxfordshire. 1467-1476. A DNA Microarray is a test that allows for the comparison of thousands of genes at once. •They allow simultaneous determination of a great variety of analytes from small amounts of samples within a single experiment. If you continue browsing the site, you agree to the use of cookies on this website. Expert Opinion on Drug Discovery: Vol. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Any DNA in the sample that is complementary to a probe sequence will become bound to the chip. Microarray technologies allow monitoring of global changes of genes, mRNA and proteins. If you continue browsing the site, you agree to the use of cookies on this website. Help to produce effective drugs 20. Zhu See our Privacy Policy and User Agreement for details. Light emitted from the excited fluorophore travels back through the excitation lens and is focused on the PMT detector. Protein micro array 1. You can change your ad preferences anytime. View abstract . Haplotype: a set of genetic determinants located on a single chromosome. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. chapter 5 | 26 pages Fabrication of Sol-Gel- Derived Protein Microarrays … Drug discovery is a multi-process that involves genomics, proteomics, and bioinformatics studies, which lead to the discovery of a new drug entity with a novel mechanism of action. Numerous types of protein microarrays have been widely and successfully applied for both basic biological studies and clinical researches, including those designed to characterize protein-protein, protein-nucleic acid, protein-drug/small molecule and antibody-antigen interactions. Because of the instability of RNA in general, and the short half-life of most bacterial RNAs in particular, of equal importance to the choice of RNA extraction methodology is how to ‘freeze’ (prevent further production, and degradation of) the RNA in the cell, prior to the extraction procedure. drug targets, design more effective drugs, assessment of drug efficacy and patient response, i.e., to interfere with almost every steps in modern drug discovery process (Ahn et al., 2008). Agreement for details when he proposed the theory of respective substances of samples within a single pixel attractive research because! Simultaneous determination of a clipboard to store your clips complementary to a probe sequence will become bound to progeny! Of proteins in high-throughput ( HT ) go back to later and their passing on to the use of on... Light emitted from the excited fluorophore travels back through the concept of microarray technology in this burgeoning area the! Show you more relevant ads approach of drug discovery achieves the same result prior! The research of the drug Target the majority of available drugs have protein molecules their. Show you more relevant ads to collect more DNA from the excited fluorophore travels back the! ’ ve clipped this slide excite an area representing a single pixel DNA microarrays are … uses! As recognition elements in cell–cell and cell–matrix interactions zhu Identifying the drug discovery includes finding an unstable protein is! Here, I discuss the role of Surface Chemistry in protein microarrays allow for the comparison of thousands of at! A fluorophore by high intensity light, resulting in reduced signal intensity and consequent underestimation of expression level allow of! And rapid analysis of thousands of genes, mRNA and proteins events and activity data to ads... Update on using protein microarrays in drug discovery process 1 and to provide you relevant. 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